The Francisella novicida transposon mutant collection
(Gallagher, et al. 2007. PNAS. 104(3):1009-14)
OBTAINING MUTANTSUpdated April 17, 2008
Strains from the F. novicida U112 transposon mutant library are now being distributed to the general research community by BEI resources. There are two mutants available for most of the nonessential genes in the U112 genome (the "two-allele" set). The strains have been single-colony purified and most have been successfully re-sequence-mapped to confirm their transposon insertion locations. Individual mutants as well as the full two-allele set (3,072 strains; catalog #NR-8024) are available for order from BEI Resources.
This site may be used to easily browse the collection and assemble your list of mutants for ordering from BEI Resources. Use the transposon insertions browser page to review and select individual insertion mutants. You will be provided with a corresponding list of BEI Resources catalog numbers which you may use to place an order with BEI Resources. To proceed, follow this link.
It is recommended that researchers verify transposon insertion locations by PCR (or sequencing) prior to use. The procedures for verifying the insertion sites by PCR are described here.
Please contact us with any comments and questions.
University of Washington
Genome Sciences, Box 355065
Foege Building, S243E
1705 NE Pacific St.
Seattle, WA 98195-5065